返回图库
生物医学
提示词描述
Roux-en-Y胃旁路术(RYGB)后，脂肪组织分布发生显著重塑，内脏脂肪/皮下脂肪比例大幅降低。皮下脂肪成为炎症因子分泌的主要来源。RYGB术后患者皮下脂肪中CD14⁺巨噬细胞显著富集，高于术前水平，且该亚群异常过度表达HMGB1。HMGB1选择性地通过巨噬细胞表面的TLR4受体促进骨髓来源巨噬细胞(BMMs)分化为破骨细胞前体(OCPs)。随后，HMGB1与OCP膜上的RAGE受体结合，促进肌动蛋白骨架重塑和肌动蛋白环形成，导致破骨细胞成熟。进一步研究表明，RAGE/TLR4可与RANKL-RANK信号通路协同作用，通过激活NF-κB和p38 MAPK通路，上调关键的破骨细胞生成基因NFATc1的表达，从而提高分化效率。为了解决这一潜在机制，本研究拟构建特异性靶向CD14⁺巨噬细胞的工程化外泌体，以精确地将HMGB1-siRNA递送至脂肪组织巨噬细胞，从而阻断异常炎症信号向骨组织的传递。
![```python
import matplotlib.pyplot as plt
import matplotlib.patches as mpatches
from matplotlib.patches import Rectangle, Ellipse, Polygon, FancyArrowPatch
import numpy as np
# Configure Chinese font
plt.rcParams['font.sans-serif'] = ['SimHei']
plt.rcParams['axes.unicode_minus'] = False
fig, ax = plt.subplots(1, 1, figsize=(10, 8))
ax.set_xlim(0, 10)
ax.set_ylim(0, 8)
ax.axis('off')
# 1. Seed germination
ax.add_patch(Rectangle((1, 6), 1.5, 0.3, color='saddlebrown', alpha=0.7, label='MS Medium'))
ax.add_patch(Ellipse((1.5, 6.5), 0.3, 0.2, color='darkgreen', alpha=0.8, label='N. benthamiana seeds'))
ax.text(1, 6.8, '1. Seeds germinate on MS medium', fontsize=10, va='bottom')
# 2. Agrobacterium inoculation
ax.add_patch(Ellipse((4, 6.5), 0.5, 0.3, color='lightblue', alpha=0.7, label='Agrobacterium suspension'))
ax.text(3.2, 6.8, '2. Agrobacterium GV3101 inoculation', fontsize=10, va='bottom')
# Arrow indicating inoculation process
arrow = FancyArrowPatch((2.5, 6.5), (3.7, 6.5),
arrowstyle='->', color='red', linewidth=2)
ax.add_patch(arrow)
# 3. Dark incubation
ax.add_patch(Rectangle((6, 5.8), 2, 1.4, color='black'
```](/_next/image?url=https%3A%2F%2Fpub-8c0ddfa5c0454d40822bc9944fe6f303.r2.dev%2Fai-drawings%2F1TlmQnMxbeuNTbpKoF6eI332tClU3piH%2F3ed380c1-9cc5-4e37-83e6-4c51fb42c6a2%2F26302c86-5b96-456e-b04b-bd25a362e066.png&w=3840&q=75)
