
APPROVED. This proposal outlines the creation of a 2D model figure, styled after Current Biology or Plant Cell graphical abstracts, to illustrate the differential ABA signaling dynamics in tomato fruit development. The model will compare the effects of dimeric (PYL2-OE) versus monomeric (PYL4-OE) ABA receptors. Layout: A horizontal timeline will represent fruit development, progressing from left (early development, low ABA) to right (late ripening, high ABA). Central elements: - X-axis: Labeled 'Fruit developmental stage (days after flowering)' with an arrow indicating increasing endogenous ABA concentration. - ABA molecule: Represented as small orange-yellow hexagons (golden #FFD700 fill, black outline, optional short side chain). - Three parallel signaling pathways (top to bottom): 1. Wild type (gray): Standard PYL receptor (red rectangle) binds ABA (orange hexagon) at a medium threshold, leading to moderate PP2C (purple circle) inhibition and normal ripening (~38–40 days). An icon of a tomato plant with medium-sized fruits will represent this. 2. PYL2-OE (dimeric, red): Two red rectangles dimerize (low basal activity), exhibiting a high ABA threshold.
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