Prompt Description

## BioRender-Style Schematic Diagram Prompts (Mitochondrial Transfer Mediated EC Transplantation) ### Overall Settings - Style: Nature sub-journal level, clear lines, bright colors, minimalist elements, highlighting core pathways and cell interactions, no redundant background. - Layout: Three-column progression (Left: Natural mitochondrial transfer; Middle: Artificial mitochondrial transplantation; Right: PINK1-Parkin mitophagy), columns connected by arrows, with the core conclusion at the top: "Mitochondrial transfer promotes EC transplantation by activating mitophagy, without functional integration." - Color scheme: MSC (green), EC (blue), mitochondria (red), autophagosome (cyan), signaling molecules (orange), text black (key molecules bolded), uniform scale. ### Left Column: Natural Mitochondrial Transfer (MSC-EC Co-transplantation) 1. Cells and Microenvironment: MSC (hCD90+) and EC (hCD31+) distributed at a 1:3 ratio, background labeled "Ischemic Stress" + red "TNF-α", bottom depicts "Collagen Hydrogel" (grid texture). 2. Transfer Structure: MSC extends 3-4 "Tunneling Nanotubes/TNTs", tubes filled with red mitochondria (arrow pointing to EC), labeled "TNFAIP2 (regulates TNT formation)" "MIRO1 (regulates mitochondrial migration)". 3. Dynamics and Effects: Label "25% of ECs acquire mitochondria within 24h", timeline (0h→7h→14h) labeled "Mitochondria present during 7h angiogenesis, disappear after 14h vascular stabilization, TNF-α can restart transfer"; upper right corner small image compares "No MSC group": EC apoptosis (Annexin-V+), no blood vessels. ### Middle Column: Artificial Mitochondrial Transplantation (No MSC Support) 1. Process and Function: Left side depicts "MSC→Separate Mitochondria→Transplant to EC (mitoAT-EC)", labeled "3:1 donor:recipient ratio"; right side EC labeled with functional indicators: "ATP↑2x" "OCR↑" "H₂O₂-induced apoptosis rate↓50%" "Scratch healing rate↑40%". 2. In Vivo Effects: Mouse hindlimb ischemia model divided into three groups—"No cell group (tissue necrosis)" "Untransplanted EC group (blood flow↓60%)" "mitoAT-EC group (human blood vessels UEA1+, blood flow↑80%)", with simplified version of laser Doppler blood flow diagram. 3. Mitochondrial Characteristics: Label "Depolarization (Ca²⁺ overload)" (JC-10 detection: green↑ red↓), "No mtDNA integration" (sequencing diagram: 7d donor mtDNA undetectable). ### Right Column: Core Molecular Mechanism (Mitophagy) 1. Autophagy Activation: Magnify local mitoAT-EC, red mitochondria wrapped by cyan "Autophagosome LC3B+", labeled "Transplanted mitochondria co-localized with autophagosomes"; pink "PINK1 (accumulates in depolarized mitochondria)" → yellow "Parkin (recruited to mitochondria)". 2. Inhibition Group Comparison: Small icon "shPINK1-M"