Prompt Description

Create a schematic diagram of a single-cell dual-omics technology workflow for nascent RNA and ATAC co-assay. Overall layout: Include screening experimental design, cell development process screening, dual-omics bioinformatics joint analysis, and expected results. Nascent RNA sequencing section: By treating cells with 4sU, metabolically label nascent RNA. After fixing the cells, perform T-to-C base conversion through IAA base conversion reaction. Determine nascent RNA and mature RNA based on mutations. ATAC section: Randomly fragment DNA using intranuclear Tn5 enzyme and perform tag ligation. Single-cell dual-omics co-assay section: Separate the nuclei of cells that have completed nascent RNA labeling and ATAC tag ligation. Separate single nuclei using water-in-oil technology. Complete RNA reverse transcription and DNA and RNA single-cell tag ligation in the water-in-oil system to achieve single-cell dual-omics co-assay. Technical focus: Research on rapid, heterogeneous biological processes with changes in chromatin accessibility, such as stem cell differentiation, T cell activation, and tumor cell development. Data analysis: Combined with AI deep learning, learn the deeper-level gene expression regulation logic of cells through joint analysis of single-cell nascent RNA and ATAC co-assay data. Color scheme: Refer to the CNS color scheme, and the color saturation should not be too high. Overall layout: Highlight the technical design section. Text: Use English only, do not mix Chinese and English.