Prompt Description

Scene 1: Rice Pistil Collection and Processing Prompt: Scientific illustration, close-up of immature rice flowers (Oryza sativa), highlighting the pistils carefully dissected under a stereomicroscope using fine tweezers. The pistils are immediately flash-frozen in liquid nitrogen, surrounded by dramatic swirling cold vapors. Style: Clean, detailed, educational, with labels. Scene 2: Tissue Grinding and Protein Lysis Prompt: Cross-sectional schematic diagram showing frozen rice pistil tissue being ground into a fine green powder with liquid nitrogen in a pre-chilled mortar. The next panel shows the powder being transferred to a microcentrifuge tube placed on ice, containing cold lysis buffer. Style: Schematic, cross-sectional view, blue and white tones to emphasize the low-temperature environment. Scene 3: Immunoprecipitation Core Process Prompt: Immunoprecipitation (Co-IP) process infographic. 1) A tube containing multiple proteins, one labeled "MEL2" (red). 2) Anti-MEL2 antibody (Y-shaped) binds to MEL2. 3) Magnetic beads (gray spheres) coated with Protein A/G capture the antibody-MEL2 complex. 4) A magnet pulls the magnetic beads, leaving other proteins in solution. 5) Elution buffer releases MEL2 and its interacting proteins (various colored shapes attached to MEL2). Style: Flat vector design, clear sequential steps. Scene 4: SDS-PAGE and Mass Spectrometry Identification Prompt: A Coomassie blue-stained SDS-PAGE gel lane showing multiple protein bands. An arrow points from the entire lane to a mass spectrometer. An inset shows a conceptual mass spectrum peak plot and a list of identified protein names (e.g., "Candidate Interacting Protein 1", "Candidate Interacting Protein 2"). Style: Realistic laboratory photograph combined with digital data overlay.