Prompt Description

I need a workflow diagram. The content is as follows: I performed sequencing on a gastric cancer cohort, with 82 patients as a test set. Based on TRG grading, they were divided into two groups: 60 cases in the R group and 22 cases in the NR group. Differential analysis (fold change > 1.5 & < 0.67, p < 0.05) yielded 191 molecules. Spearman analysis identified 241 molecules significantly correlated with TRG. Additionally, molecules with a frequency greater than or equal to 20% in either NR or R were selected. The overlap of molecules obtained from these three strategies resulted in 73 molecules. Subsequently, a prediction model was constructed using logistic regression and 10-fold cross-validation. Another cohort was used as a validation set, and the AUC performance was good. Please draw a workflow diagram in the style of high-impact CNS journals, suitable for direct publication in a paper.