Prompt Description

“After obtaining positive strains, large-scale fermentation culture is carried out. Recombinant insulin production typically employs high-density fermentation. Temperature, pH, and dissolved oxygen are controlled in the fermenter, and nutrients are provided to promote cell growth. The induction timing can be adjusted during the fermentation process according to the expression vector design: for example, IPTG is added to induce expression when the cell density reaches a certain level, or a promoter self-control expression system is used. Regulation strategies affect the final yield. Using a professional bioreactor can control the sterile environment and parameters to maximize yield. Literature reports that E. coli fermentation can produce hundreds of milligrams of insulin chains per liter of culture medium. In addition, fermentation control can reduce the production of inhibitors and improve the yield and purity of recombinant proteins.”