Prompt Description

1. Extract DNA from leaves. 2. Perform PCR on T0 plants using primers 35S_F and Dm1_Cas9_304_R. 35S_F: targets the cauliflower mosaic virus 35S promoter, which drives the expression of the SpCas9 gene and selectable marker gene on the CRISPR vector. Dm1_Cas9_304_R: targets a specific sequence within the SpCas9 gene. 3. A positive result indicates that the plant is a true transformant and not a false positive that escaped resistance screening.