Title: Superior Output and Molecular Evidence: From Efficient Assembly to Chemical Imprint Design Concept: Employ a central radial layout, with the "expanded endoplasmic reticulum" as the core, showcasing its output in four dimensions: yield, quality, mechanism, and evidence. Central Figure: A simplified, highlighted, and enlarged schematic diagram of the endoplasmic reticulum (ER), with simplified diagrams of proteins folding inside. Annotate it with "Expanded ER: Optimized Production Workshop." Four extending modules around it (connected to the central ER with arrows): Module 1 (Top Left): Yield and Assembly Quality Icons: Bar graph icon + protein multimer icon. Key Results: ELISA: Functional antibody yield increased several-fold (e.g., 3.7-4.3 times). SEC: The proportion of fully assembled (multimeric) antibodies significantly increased. Conclusion: High Yield & High Quality Module 2 (Top Right): Intracellular Processes and Molecular Environment Icons: Hourglass (time) icon + molecular chaperone (BiP) icon. Key Results: Subcellular fractionation: Antibody residence time in the ER is prolonged. Immunoblotting: Endoplasmic reticulum chaperone protein BiP accumulation level is higher. Conclusion: Ample Time & Enhanced Assistance Module 3 (Bottom Left): Detailed Chemical Evidence Icons: Mass spectrometer icon + schematic diagram of high-mannose glycan structure. Key Results: Mass spectrometry glycosylation analysis: The proportion of high-mannose N-glycans on antibodies is significantly increased. Conclusion: Direct "Molecular Imprint" of ER Retention Module 4 (Bottom Right, can serve as a summary module): Title: Systematic Verification Closed Loop Content: Use a circular arrow diagram to briefly show: Structural Expansion → Time/Environment Optimization → High Yield and High Quality → (Glycan Evidence Confirmation). Final Conclusion: A complete and self-consistent evidence system is formed from the basic structure to the final product, demonstrating the comprehensive advantages of the engineered production platform.
