Prompt Description

Title: Superior Output from Engineered "Cell Factories": From Increased Yield to Molecular Imprints Design Concept: Employ a "top-down" or flow-chart layout to showcase the functional advantages and detailed validation resulting from ER expansion. Top Overview/Central Image: A simplified schematic of a "cell factory": The center features a prominently highlighted, enlarged endoplasmic reticulum (ER), labeled "Expanded ER." Three arrow paths extend from it, each pointing to one of the three result modules below. Three Result Modules Arranged Side-by-Side: Module 1: Increased Yield and Quality Icons: Test tube/bar graph icon + protein complex icon. Core Data/Figure: A concise bar graph illustrating ELISA results: Antibody production in engineered strains (colored bars) is several times higher than in WT (gray bar). A concise SEC chromatogram comparison: The multimer main peak is higher and sharper in engineered strains (solid line). Concluding Text: Yield ↑ & Assembly Quality ↑ Module 2: Intracellular Trafficking and Molecular Environment Icons: Organelle (ER, vacuole) schematic + molecule (BiP) icon. Core Data/Figure: A simple flowchart: Antibodies reside longer in the "Expanded ER" (clock icon), and then some enter the "vacuole" (arrow indicates). An immunoblot band comparison (based on Figure 4b): Engineered plant bands show a stronger BiP signal. Concluding Text: ER Residence ↑ & Optimized Folding Environment Module 3: Detailed Chemical Evidence Icons: Simplified mass spectrometer diagram + glycan structure icon. Core Data/Figure: A pie chart comparison: The left pie chart represents WT antibody glycoforms, with complex-type (blue) predominating; the right pie chart represents engineered plant antibody glycoforms, with a significantly increased proportion of high-mannose-type (orange). (Or use a simplified version of Figure 3b from the literature, showing changes in the proportion of glycoforms at specific glycosylation sites). Concluding Text: High-Mannose-Type Glycans ↑ (Chemical Imprint of ER Retention) Bottom Final Summary Box: Text: Conclusion: Through host engineering, we have constructed a highly efficient protein production platform with "expanded space, ample time, and sufficient assistance," achieving global optimization from structure to function.