Prompt Description

Step 1: Platform Selection (LAB strain) Objective: To select a well-characterized "chassis" strain known for high productivity and ease of manipulation, ensuring maximal efficiency in subsequent modifications. Technical Advantage: Leveraging the latest complete genome and multi-omics data, we upgrade the traditional empirical "user-friendly" platform into a digitized model system with transparent information, enabling precise design. (ER Morphology Comparison Chart) - Visually demonstrates the direct effects and core principles of ER engineering (platform modification). Step 2: Target Identification (CCT gene) Objective: To identify the key switch controlling the size of the "production workshop" (endoplasmic reticulum). Enhancing SIgA production involves optimizing the environment for its folding and assembly rather than directly amplifying its gene. Technical Advantage: Demonstrates the capability of rational design. Based on the clear cell biological principle of "membrane synthesis-ER expansion," bioinformatics is used to precisely locate key regulatory genes from massive genomes, avoiding blind screening. (Target and Mutant List) - Clearly lists three specific target genes (CCT1A, CCT1B, CCT2) and their gene IDs, and displays mutants with different combinations. Step 3: "Scissors" Design (gRNA) Objective: To perform precise "gain-of-function" rather than "loss-of-function" editing on the target. The aim is to allow cells to continuously produce more membrane components, thereby naturally expanding the ER, rather than killing the cells. Technical Advantage: Represents the precision and programmability of CRISPR/Cas9 gene editing technology. Designing gRNAs to cut between specific domains achieves "fine-tuning" of protein function, which is difficult to achieve with traditional transgenic overload or gene knockout techniques. (Gene Editing Sequencing Verification Chart) - Confirms the precision and effectiveness of CRISPR editing in the form of "molecular fingerprints."