Layout Suggestions: Title: Genetically Engineered Plant Cell Factories: From Structural Remodeling to Functional Output Core Logic Flow (arrows to guide the eye): Gene Editing → Structural Change → Increased Yield and Quality Content Sections: Top Left: Genetic Engineering Technology and Structural Verification (Cause and Form) Figure: Side-by-side CLSM images (tubular ER in WT on the left, lamellar ER in mt1 on the right, arrows indicating changes). Figure caption: "Fig 1e: CRISPR/Cas9 editing leads to ER structural remodeling." Text: Core genetic engineering technology: CRISPR/Cas9 editing of the CCT gene to relieve self-inhibition. Conclusion: ER network density doubles, providing an expanded "workshop" for protein production. Top Center: Biochemical Basis and Quantitative Results (Quantity and Quality) Figure: Use a combination of bar graphs. First set of small figures: "Lipid analysis" results showing a 60% increase in PC content. Second set of small figures: "ELISA" results bar graph showing that the engineered strain (colored bars) has significantly higher antibody production than WT (gray bars). Text: Increased membrane lipid synthesis → Significant increase in antibody production (up to 4.3 times). Top Right: Quality Assessment and Secretion Verification (Quality and Outcome) Figure: Use chromatogram + schematic diagram. Top: "SEC chromatogram" showing that the proportion of the polymer main peak area of the engineered strain (solid line) is greater than that of WT (dashed line). Bottom: "Extracellular fluid extraction" schematic diagram (leaf → centrifugation → collect liquid), accompanied by a simple bar graph showing higher extracellular antibody content. Text: More complete antibody assembly → More functional antibodies secreted into the extracellular space. Bottom: Core Conclusion Box Text: Conclusion: Through CRISPR-mediated endoplasmic reticulum engineering, we have successfully transformed plants into efficient antibody production platforms, achieving a global enhancement of "structural expansion - yield increase - quality optimization - effective secretion". Visual: Highlight with a prominent border. Design Style: Maintain a simple scientific blue/green color scheme, unify the style of all charts, clear arrows and logic flow, and concise text.
The constructed recombinant plasmid is introduced into E. co...