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Schematic diagram of remote ischemic preconditioning. | SciDraw Gallery

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Schematic diagram of remote ischemic preconditioning.

Biomedical

Prompt Description

Schematic diagram of remote ischemic preconditioning.

Related Illustrations

1. Core Scene Depiction

Generate a schematic diagram in a landscape format with a scientific vector illustration style, clearly showing the working principle of a modular CRISPR-Cas9 gene editing system for plants.

2. Detailed Scene Description (by module)

Module 1: Plasmid Vector (pDV003 - Left)

Draw a large circular plasmid. Label the "Pol II promoter (CmYLCV)" module on the ring, extending a long horizontal RNA chain (polycistronic transcript) from it.

On the RNA chain, highlight two target sequences in bright red and bright orange, labeled as "gRNA: G5" and "gRNA: G4.2" respectively.

Each target sequence is flanked by unique cleavage markers (such as diamonds or keyholes), representing "Csy4 recognition sites."

Below the plasmid ring, draw a separate "reporter gene expression cassette" containing the "FMV34S promoter" and "chloroplast-targeted mCherry" icons.

Label the "AarI site & ccdB negative selection gene" region at the bottom of the plasmid.

Module 2: Csy4 Processing and gRNA Maturation (Middle)

Next to the RNA chain, draw a blue or green "molecular scissors" shaped protein, labeled as "Csy4 ribonuclease."

Clearly show Csy4 cutting at the two recognition sites, precisely cleaving the long RNA chain to release two independent, complete single-stranded gRNAs.

Module 3: sgRNA Structure Optimization Close-up (Middle - Magnifying Glass Frame)

Add a magnifying glass-style close-up frame to one of the released gRNAs.

Inside the frame, compare side-by-side:

Left (Standard): A simple short stem-loop structure, with the note "Standard sgRNA" and sequence "TTTT."

Right (Optimized): A structure with a significantly longer stem-loop, with the note "Optimized sgRNA (Dang et al.)." Indicate "double-stranded region extension (+5 bp)" and "key mutation (T→C)" with arrows and text.

Module 4: Cas9 Complex and Gene Editing (Right)

Draw a clear structural model of the SpCas9 protein (multi-domain assembly).

Show an optimized gRNA binding to Cas9 to form a "Cas9/gRNA ribonucleoprotein complex (RNP)."

Draw two parallel blue genomic DNA double helices, labeled with target points "Genomic Locus G5" and "Genomic Locus G4.2".

Show the Cas9 complex binding to the DNA target and creating a "DNA double-strand break (DSB)" upstream of the PAM sequence (indicated by a break or lightning symbol).

3. Style and Visual Requirements

Overall style: Simple, semi-realistic scientific vector illustration with clear lines and precise structure.

Color scheme: Use academic journal color schemes. It is recommended to use blue for DNA and RNA.

1. Core Scene Depiction Generate a schematic diagram in a l...

Agrobacterium-mediated transformation has become the most widely used method for stable genetic transformation of wheat. It results in low transgene copy numbers and more predictable insertion patterns, leading to stable inheritance and reliable gene expression.

Agrobacterium-mediated transformation has become the most wi...

Create a visually compelling graphical abstract illustrating a two-stage electro-assisted dark fermentation-microbial electrolysis cell (DF-MEC) system for biohydrogen production. Employ flat scientific icons, a soft color palette, prominent arrows, and minimal text.

Panel 1 – Feedstock (left):
Icons: Food waste and swine manure.
Arrow leading to a mixed tank.
Text (small):
Carbon-rich + Nitrogen-rich → Balanced feed
Tags: Self-pH regulation | Optimized C/N ratio | Electron-rich
Side icons:
• Heat treatment: 80 °C, 20 min
• Suppressed methanogenesis (crossed-out CH₄ icon)

Panel 2 – Stage I: Electro-Assisted Dark Fermentation (center):
Single fermenter vessel containing microorganisms in broth.
Electrodes inserted:
• Carbon felt bioanode
• Stainless steel biocathode
Connected to an external power supply.
Illustrate electron flow (e⁻): anode → circuit → cathode, with electrons interacting with microbes.
Side icons:
• Hydraulic Retention Time (HRT) control
Text (short):
Electro-DF | Redox control
Inset zoom 1 (cell level):
Microbial cell diagram showing:
NADH/NAD⁺ ratio increase → Hydrogenase activity increase → H₂ production increase
Arrow from anode to cell, labeled "Low ORP"
Fade out lactate/ethanol icons (representing reduced production).
Emphasize enhanced extracellular electron transfer (EET).
Output arrow:
Acetate-rich effluent

Create a visually compelling graphical abstract illustrating...

A training dataset from the GEO database GSE46922 was used to identify differentially expressed genes (DEGs) intersecting with ferroptosis-related genes. These DEGs were then subjected to PPI network analysis, KEGG pathway enrichment analysis, immune infiltration analysis, and immune checkpoint analysis. Finally, the expression levels of the DEGs were validated in the GEO database GSE23754 validation set using box plots.

A training dataset from the GEO database GSE46922 was used t...