Prompt Description

1. Layout & Style: Structure: Vertical flowchart layout, divided into a left-side "navigation bar/stage label" and a right-side "main content area." Color Scheme: Main Color: Blue tones (medical/biotech style). Left Labels: Dark blue rounded rectangles with white text. Right Content Background: Light blue background with gradient blue or solid blue boxes inside, white text. Lines and Arrows: Thin solid blue lines. Section Division: The chart is divided into five logical parts from top to bottom: Top Title Phenomenon Observation Mechanism Exploration Recovery Verification Research Significance 2. Detailed Content & Logical Connections: Please construct nodes and connections in the following order: Top: Overall Title Location: Topmost, spanning the entire chart. Text: "Mechanistic study of PPARγ protein SUMOylation-induced microglia metabolic reprogramming interfering with central amygdala circuit remodeling leading to social deficits in autism" Style: Dark blue background, large white font. Part 1: Phenomenon Observation Left Label: Phenomenon Observation Right Content: Divided into two parallel sub-modules. Sub-module 1 (Left): Title: Research Content 1: Individual Level Core Node: SD pregnant rats Treatment: LPS/NS injection (pointing to SD pregnant rats) Outputs (arrows branching downwards into 6 results): Social behavior detection PPARγ protein SUMOylation HK2 expression upregulation Microglia metabolic reprogramming Microglia phenotype polarization Microglia synaptic pruning impairment Central amygdala circuit remodeling abnormality Sub-module 2 (Right): Title: Research Content 2: Cellular Level Core Node: Microglia Treatment: LPS/NS treatment (pointing to microglia) Outputs (arrows branching downwards into 5 results): PPARγ protein SUMOylation HK2 expression upregulation Microglia metabolic reprogramming Microglia phenotype polarization Microglia synaptic pruning impairment Part 2: Mechanism Exploration Left Label: Mechanism Exploration Right Content: Title: Research Content 3: Molecular Level Starting Node: Microglia Branching Process (four parallel mechanism verifications): PIAS1 cascade initiation verification PPARγ signaling transduction verification HK2 metabolic hub function verification Metabolic reprogramming phenotype-driven function verification Convergence Node: The arrows from the above four branches point to a general box below: Biological detection. Conclusion Box (text within dashed box): "Precisely intervene at different nodes from the molecular level to elucidate microglia"