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Lats1-AKO and Lats1^f/f^ mice were fed a high-fat diet fo... | SciDraw Gallery

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Lats1-AKO and Lats1^f/f^ mice were fed a high-fat diet for 12 weeks, followed by local injection of AAV-BST2 into subcutaneous adipose tissue. The high-fat diet was continued until week 24. Please draw a schematic diagram of the experimental design.

Biomedical

Prompt Description

Lats1-AKO and Lats1^f/f^ mice were fed a high-fat diet for 12 weeks, followed by local injection of AAV-BST2 into subcutaneous adipose tissue. The high-fat diet was continued until week 24. Please draw a schematic diagram of the experimental design.

Related Illustrations

$Network Diagram for Early Warning of Clinical Emergencies Due to Imbalances in Intracellular Ion Homeostasis. Core concept: To reveal how imbalances in seven core ions (Na⁺, K⁺, Ca²⁺, Cl⁻, H⁺/pH, Mg²⁺, NH₄⁺) can trigger multi-system chain reactions, ultimately manifesting as specific clinical emergencies. I. Five-Layer Vertical Structure Top Layer - Ion Projection Source: Seven color-coded source points surrounding the human body, representing seven ions, emitting colored translucent beams. Middle Layer - Human System Outline: A neutral gray human anatomical diagram, receiving all ion beams as an "interaction battlefield." Lower Layer - Comprehensive Emergency Area: Five red warning boxes listing ultimate critical conditions such as malignant arrhythmias, acute brain injury, neuromuscular crisis, acute kidney injury, and multiple organ dysfunction syndrome (MODS). Bottom Layer - Symptom Vignette Column: Six grids, each displaying typical diseases and characteristic painful postures of patients caused by ion imbalances. II. Key Points of the Seven Major Ion Networks Na⁺ (Dark Blue): Osmotic pressure dominance, the beam mainly projects to the brain (cerebral edema/atrophy) and the whole body. K⁺ (Magenta): Cardiac electrophysiological activity arbiter, the beam connects the heart (arrhythmia) and muscles (weakness/paralysis). Ca²⁺ (Orange): Muscle contraction switch, the beam connects neuromuscular (spasm/weakness), heart (QT changes), and kidney (stones). H⁺/pH (Purple): Enzyme activity lifeline, radial beams connect all organs, leading to widespread dysfunction. Cl⁻ (Green): Low-key coordinator, the beam emphasizes its association with Na⁺ (electrical neutrality) and pH (acid-base balance). Mg²⁺ (Cyan): Ion channel gatekeeper, the beam connects the heart (torsades de pointes) and neuromuscular system, and regulates K⁺ and Ca²⁺ with dashed lines, with a warning note: "Refractory imbalance? Check blood magnesium first!" NH₄⁺ (Brown): Liver-kidney-brain axis toxin, the beam originates from the liver, passes through the kidney, and the core path directly penetrates the blood-brain barrier towards the brain (hepatic encephalopathy). III. Bottom Symptom Vignette Column (Key Clinical Anchors) Presenting typical painful postures with minimalist stick figures, intuitively connecting mechanisms with bedside manifestations: Hyperkalemia: Supine stick figure, ECG showing cardiac arrest → "Premonition of cardiac arrest." Hyponatremic Cerebral Edema: Stick figure holding head with both hands, expressing pain → "Explosive headache." Hypocalcemic Tetany: Stick figure with forearm in "obstetrician's hand" position → "Carpal spasm." Metabolic Acidosis: Forward-leaning sitting posture, deep and rapid breathing → "Kussmaul breathing, fatigue." Arrhythmia/Heart Failure: Stick figure clutching chest and curling up, expressing extreme pain → "Palpitations, chest pain." Kidney Failure/Stones: Bending over and clutching waist, expressing pain → "Waist pain, anuria." IV. Global Warning and Value Top Warning: "Seven Signs of Chemical Life: Any imbalance can trigger a systemic domino effect. Time window: Minutes to hours." This diagram achieves a complete narrative loop from ion mechanisms to organ interactions and then to specific symptoms.$

Network Diagram for Early Warning of Clinical Emergencies Du...

1. Overall Layout:
A centered circular closed loop, equally divided into four quadrants. Four segments are equidistantly distributed clockwise on the ring, connected by light blue arrows, forming a complete cycle.

2. Four Core Segments (clockwise):

First Segment (12 o'clock position): Agricultural Waste Input

Icon: A simple corn cob outline icon.

Label: "Agricultural Waste" (e.g., corn cobs).

Visual: The icon is filled with light green, symbolizing nature and raw materials.

Second Segment (3 o'clock position): Biomanufacturing Core

Icon: A combination icon of a flask and a double helix DNA, representing synthetic biology fermentation.

Label: "L-Lactic Acid Production."

Visual: The icon is filled with blue, symbolizing technology and manufacturing.

Third Segment (6 o'clock position): Material Synthesis

Icon: A polymer chain structure icon composed of "PLA" letters.

Label: "PLA Synthesis."

Visual: The icon is filled with dark blue, symbolizing materials and polymerization.

Fourth Segment (9 o'clock position): End Products

Icon: An outline icon of a degradable lunch box or shopping bag.

Label: "Green End Products."

Visual: The icon is filled with green, symbolizing environmental protection and finished products.

3. Connections and Dynamics:

Each segment is connected by simple curved arrows, with the arrow color being light blue, indicating the direction of material and value flow.

In the center of the ring, a circular arrow icon or the words "Green Material Ecology" can be added to highlight the theme.

4. Style and Color Scheme:

Background: Pure white.

Lines: All graphics use thin solid line borders, without shadows, maintaining a flat design.

Color Scheme:

Green (#4CAF50): Represents agriculture and environmental protection.

Blue (#2196F3): Represents technology and manufacturing.

Light Blue (#87CEEB): Used for connecting arrows.

Font: Use a sans-serif font (such as Microsoft YaHei, Arial), with small and clear label text.

1. Overall Layout: A centered circular closed loop, equally ...

I want to create a simplified flowchart outlining the following steps: single-cell sequencing of corneal repair in rodents and non-human primates; cross-species analysis of cell type homology; and identification of differentially expressed genes in the early stages of the repair process.

I want to create a simplified flowchart outlining the follow...

FGF1 knockout mice exhibit impaired cognitive function. (1) Spontaneous alternation rate and total arm entries in the Y-maze test were significantly decreased in FGF1-/- mice. In the passive avoidance test, the time spent in the dark chamber on the second day was significantly reduced in FGF1-/- mice. The novel object recognition test indicated impaired short-term memory in FGF1-/- mice. Behavioral experiments demonstrated a decline in cognitive abilities in FGF1-/- mice compared to the wild-type group. (2) Transcriptomic analysis of the hippocampus revealed that FGF1 knockout leads to complex disorders in the nervous system, immune system, and lipid metabolism. (3) Lipidomic analysis of the hippocampus suggested lipid remodeling after FGF1 knockout, and related network analysis indicated that ceramide Cer(d18:1/22:0) plays a central role in related glycolipid metabolic pathways. (4) FGF1-/- mice showed activation of brain inflammatory responses, activation of the NF-κB/NLRP3 neuroinflammatory pathway, increased expression of brain inflammatory factors (IL-1β, TNF-α, IL-6), and impaired antioxidant stress function. Simultaneously, the blood-brain barrier was damaged, and peripheral immune cells invaded the central nervous system. (5) Neuronal expression was decreased and neuronal synaptic function was impaired in FGF1-/- mice. (6) Myelin regeneration function was impaired in FGF1-/- mice.

FGF1 knockout mice exhibit impaired cognitive function. (1) ...