Prompt Description

Figure Design Proposal: Integrated Flowchart Figure Title: Integrated Computational and Experimental Strategy for Identifying Upstream Transcriptional Regulators of Fgf4 Design Concept: Integrates a "left-to-right process narrative" with a "top-to-bottom results presentation," merging strategy and results. Employs professional colors, high information density, and clear visual guidance. Detailed Explanation and Enhancement Points for Each Module: Left Side - Strategy Flowchart Area Design: Use grayscale or low-saturation color blocks and arrows to clearly define steps, reflecting the methodology. Add small icons (such as a DNA double helix or magnifying glass) next to the "MEME Prediction" and "TOMTOM Comparison" steps to enhance recognition. Key Points: Indicate key parameters, such as sequence range, MEME's E-value threshold, and the name of the database used. Middle - Results Visualization Area Top (MEME Result Display): Sequence Logos: Display the predicted Top 3 motifs side-by-side in high-definition, colorful sequence logo format. This is the core result of the MEME analysis and must be visually appealing. Annotation: Clearly label the MEME E-value and width below each logo. Bottom (TOMTOM Results and Filtering): Bar Chart: Plot the top 3-5 candidate transcription factors with the highest significance after TOMTOM comparison as a horizontal bar chart, using their matching -log10(p-value) as the metric. Design: Use professional color schemes (such as viridis or Set2 color palettes). Arrange bars in descending order from left to right by value, and directly label the factor name (e.g., KLF5) and specific p-value at the end of the bar. Filtering Path: To the right of the bar chart, use a funnel icon or filtering icon to point to the final 1-2 "core candidate factors," highlighting them with different colors or star markers. Right Side - Experimental Validation Bridge Area Design: Delineate with dashed boxes or light-colored backgrounds to indicate this is the next step guided by prediction. Content: Briefly illustrate subsequent key validation experiments with icons and text, such as ChIP-qPCR (magnetic beads and DNA icons) and reporter gene assays (luciferase icon), with arrows pointing to "validated regulators." Function: This module greatly enhances the scientific integrity and depth of the figure, indicating that the research goes beyond computational prediction and completes closed-loop validation.