Prompt Description
1. In vitro CC50, IC50, and plaque assays of the HSP90 inhibitor AUY922 demonstrate its antiviral activity against GCRV. 2. Pre-incubation of cells with AUY922 validates its ability to prevent GCRV infection by inhibiting the expression of genes related to cell membrane receptors, inflammation, and apoptosis. 3. Co-incubation of cells with AUY922 validates its anti-GCRV activity by inhibiting the expression of genes related to cell membrane receptors, inflammation, and apoptosis. 4. Post-incubation of cells with AUY922 validates its therapeutic effect against GCRV infection by inhibiting the expression of genes related to cell membrane receptors, inflammation, and apoptosis. 5. Cell-based assays using inflammation and apoptosis agonists confirm that AUY922 exerts its antiviral activity by inhibiting the expression of inflammation- and apoptosis-related genes. 6. Individual-level validation using techniques such as immunofluorescence, RT-PCR, Western blotting, electron microscopy, and immunohistochemistry confirms that AUY922 inhibits the expression of genes related to cell membrane receptors, inflammation, and apoptosis, thereby exerting its anti-GCRV activity.
![Subject: Experiment Flowchart of Bacterial Activation and Phage Treatment of Biofilm Formation
Style: Concise, scientific experimental flowchart, black lines on a white background, clear icons
Requirements:
Use clear arrows to connect each step.
All key nouns must be represented by icons/pictures, and ensure the icons are easy to understand.
The process layout is from left to right and from top to bottom, with clear logic.
Steps and Icon List:
Start
[Icon: Stock tube] (containing target bacteria)
Use [Icon: Inoculation loop] to pick up bacterial solution from [Icon: Stock tube].
Perform [Icon: Streak plating] on [Icon: 90mm petri dish] (containing solid medium) to activate bacteria.
Incubate in an incubator until [Icon: Single colony] grows.
Use [Icon: Inoculation loop] to pick a [Icon: Single colony].
Inoculate the picked colony into [Icon: Erlenmeyer flask] containing [Icon: 2216E liquid medium] and shake culture.
Measure periodically until [Icon: Spectrophotometer] shows the bacterial solution [Icon: OD600=0.1].
Branch point: Dispense the bacterial solution into two new, sterile [Icon: Erlenmeyer flasks].
Control group: Keep one [Icon: Erlenmeyer flask] as is.
Experimental group: Add [Icon: Bacteriophage] to the other [Icon: Erlenmeyer flask].
Use [Icon: Pipette] to transfer the liquid from the two Erlenmeyer flasks to the wells of 4 [Icon: 24-well plates] respectively (the liquid from each Erlenmeyer flask corresponds to 2 well plates).
Parallel processing: Place these 4 [Icon: 24-well plates] in [Icon: Different environments] (e.g., different temperatures or shakers) and incubate for [Icon: 24 hours].
After incubation, remove all well plates.
Collect the [Icon: Biofilm] formed in each well plate.
Place the collected [Icon: Biofilm] samples in [Icon: 1.5ml centrifuge tube].
End (for subsequent analysis).
Layout: Please ensure that steps 1-7 are arranged vertically, and after step 8 (dispensing), the control group and the experimental group expand downwards in parallel, and steps 9-13 converge again.](/_next/image?url=https%3A%2F%2Fpub-8c0ddfa5c0454d40822bc9944fe6f303.r2.dev%2Fai-drawings%2FtBg6VEIvj0JHqBd0LOLXjlnAkPj8Lpax%2F06996af5-ce83-4761-bc4c-8fa3feecafab%2F4cedc944-94ba-4056-b4c9-a7ad54eefabf.png&w=3840&q=75)
