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![Three-stage progressive research: from basic mechanism analysis → novel molecule design → formulation development and application verification, with each stage building upon the previous, ultimately achieving efficient skin vitrification cryopreservation.
Stage 1: Analysis of the Structure-Activity Relationship and Regulatory Mechanism of Vitrification Agents
Core Objective: To elucidate the "structure-property" relationship and molecular synergistic mechanism of vitrification agents.
Research Content and Methods:
Basic Characterization of Vitrification Performance: Determine the critical vitrification concentration and analyze vitrification transition characteristics using differential scanning calorimetry.
Molecular Mechanism Simulation: Computer simulation (molecular structure optimization, energy minimization, electrostatic potential distribution, interaction energy calculation, hydration and water molecule residence time analysis).
Stage Output: Model of the structure-activity relationship and synergistic regulatory mechanism of vitrification agents.
[Suggested Illustration]: Molecular structure model + schematic diagram of energy/hydration interaction
Stage 2: Design and Synthesis of Novel Vitrification Molecules Based on Structure-Activity Relationship
Core Objective: To establish a novel vitrification molecule design strategy and obtain high-performance candidate molecules.
Research Content and Methods:
Molecular Design and Synthesis: Design and chemically synthesize novel vitrification molecules based on the structure-activity relationship from Stage 1.
Structure and Performance Verification: Characterize the molecular structure using infrared spectroscopy, nuclear magnetic resonance (hydrogen/carbon NMR), and high-resolution mass spectrometry; test its vitrification performance (critical cooling/heating rate) and ice crystal inhibition ability (ice nucleation/growth, recrystallization inhibition).
Stage Output: Candidate molecules with excellent vitrification and ice crystal inhibition properties.
[Suggested Illustration]: Molecular design flowchart + structural characterization spectra + microscopic images of ice crystal inhibition
Stage 3: Development of Efficient Cryoprotectant Formulations and Verification of Skin Cryopreservation Effect
Core Objective: To optimize cryoprotectant formulations, establish a skin vitrification cryopreservation protocol, and verify its effectiveness.
Research Content and Methods:
Formulation and Process Optimization: Optimize cryoprotectant formulations based on the candidate molecules from Stage 2; test the permeability of protective agents and develop loading/unloading protocols.
Skin Cryopreservation and Evaluation: Design a skin vitrification cryopreservation procedure and evaluate the cryopreservation effect through cell viability tests, histological staining, and mechanical property analysis.
Stage Output: Efficient vitrification cryoprotectant formulation and optimized skin cryopreservation protocol.
[Suggested Illustration]: Schematic diagram of formulation optimization + skin tissue sections + mechanical property curves
Progressive Relationship
Stage 1 provides the "structure-property" design basis for Stage 2, Stage 2 provides the core functional molecules for Stage 3, and Stage 3 verifies the application effect of the entire process, forming a "mechanism-design-application" closed loop.
Illustration Style: Clear flowchart, with three stages distinguished by color, arrows indicating progressive logic, and key nodes accompanied by simplified schematic diagrams.](/_next/image?url=https%3A%2F%2Fpub-8c0ddfa5c0454d40822bc9944fe6f303.r2.dev%2Fai-drawings%2FXYJ0h2vTvP0v0DQtTMWj2YH0O11qgSqi%2Fdba97ac9-3cf1-42b8-9a8e-4074ebebd70b%2Fec20b4ed-f083-4abc-91f0-d73d16ffb130.png&w=3840&q=75)
Please generate a schematic diagram illustrating the mechanism for a biomedical master's thesis, with a professional, concise, and logically clear style. Adopt a left-to-right or center-out layout, distinguishing between "diabetic injury" and "therapeutic intervention" sections. Suggested visual elements and layout: Left side (Injury side): Background labeled "HG", with a dark tone (e.g., light gray). Draw a simplified diagram of a vascular endothelial cell. Above or inside the cell, use "↓" symbols and dashed arrows to indicate two inhibited pathways: Pathway 1 (Top): Labeled "Hippo pathway inhibition", key molecular changes: P-YAP ↓ → YAP nuclear translocation ↑ → leading to "Inflammatory response ↑" (represented by an explosion icon, labeled IL-1β, IL-6, TNF-α ↑). Pathway 2 (Bottom): Labeled "Wnt/β-catenin pathway inhibition", key molecular changes: β-catenin ↓ → CyclinD1 ↓. Below the cell, use downward arrows and crosses to indicate cellular functional consequences: Cell proliferation ↓, Cell migration ↓, VEGF/PCNA ↓. Center (Intervention and turning point): Place a prominent bottle or container icon in the center, labeled "MWE-A", and use a thick green solid arrow pointing to the cell on the right to indicate therapeutic intervention. Right side (Therapeutic recovery side): Background labeled "MWE-A treatment", with a bright tone (e.g., light blue or green). Draw a simplified diagram of a healthy, active endothelial cell. Inside the cell, use "↑" symbols and solid arrows to indicate two activated pathways: Pathway 1 (Top): Labeled "Hippo pathway activation", key molecular changes: P-YAP ↑ → YAP nuclear translocation ↓ → leading to "Inflammatory response ↓" (inflammation icon reduced or disappeared). Pathway 2 (Bottom): Labeled "Wnt/β-catenin pathway activation", key molecular changes: β-catenin ↑ → CyclinD1 ↑. Below the cell, use upward arrows to indicate functional improvement: Cell proliferation ↑, Cell migration ↑, VEGF/PCNA ↑. On the right side of the cell, draw a simplified diagram of the skin wound healing process: A wound that is closing. Draw newly formed capillaries inside the wound (labeled CD31↑). Use icons to represent increased collagen fibers and epithelial layer coverage. Style requirements: Flat or minimalist line style, professional color scheme (e.g., use blue/green to represent activation/benefit, red/gray to represent inhibition/injury). All molecule names, pathway names, arrows, and change trends (↑↓) must be clearly distinguishable. Ensure that causal arrows point clearly, and the overall layout is well-spaced.
Three-stage progressive research: from basic mechanism analy...
