Prompt Description
Generate a plasmid DNA backbone comparison diagram. On the left, depict a traditional plasmid backbone as a circular structure, labeled 3000-5000 bp, featuring an antibiotic resistance gene (highlighted in red), an oriC replication origin, and a promoter region, with a gray-orange gradient fill. On the right, illustrate a MiniPlasmid-Pro-1 ultra-compact backbone as a circular structure, labeled 600 bp, containing only a RelB small RNA expression unit (in blue), a replication origin, and a promoter, with a blue-green gradient fill. Connect the two backbones with a bold arrow labeled "85-90% size reduction" and "60-75% cost reduction". The background should be pure white, in a molecular biology textbook style, with a flat design, no shadows, and vector graphic quality.
Related Illustrations
![Three-stage progressive research: from basic mechanism analysis → novel molecule design → formulation development and application verification, with each stage building upon the previous, ultimately achieving efficient skin vitrification cryopreservation.
Stage 1: Analysis of the Structure-Activity Relationship and Regulatory Mechanism of Vitrification Agents
Core Objective: To elucidate the "structure-property" relationship and molecular synergistic mechanism of vitrification agents.
Research Content and Methods:
Basic Characterization of Vitrification Performance: Determine the critical vitrification concentration and analyze vitrification transition characteristics using differential scanning calorimetry.
Molecular Mechanism Simulation: Computer simulation (molecular structure optimization, energy minimization, electrostatic potential distribution, interaction energy calculation, hydration and water molecule residence time analysis).
Stage Output: Model of the structure-activity relationship and synergistic regulatory mechanism of vitrification agents.
[Suggested Illustration]: Molecular structure model + schematic diagram of energy/hydration interaction
Stage 2: Design and Synthesis of Novel Vitrification Molecules Based on Structure-Activity Relationship
Core Objective: To establish a novel vitrification molecule design strategy and obtain high-performance candidate molecules.
Research Content and Methods:
Molecular Design and Synthesis: Design and chemically synthesize novel vitrification molecules based on the structure-activity relationship from Stage 1.
Structure and Performance Verification: Characterize the molecular structure using infrared spectroscopy, nuclear magnetic resonance (hydrogen/carbon NMR), and high-resolution mass spectrometry; test its vitrification performance (critical cooling/heating rate) and ice crystal inhibition ability (ice nucleation/growth, recrystallization inhibition).
Stage Output: Candidate molecules with excellent vitrification and ice crystal inhibition properties.
[Suggested Illustration]: Molecular design flowchart + structural characterization spectra + microscopic images of ice crystal inhibition
Stage 3: Development of Efficient Cryoprotectant Formulations and Verification of Skin Cryopreservation Effect
Core Objective: To optimize cryoprotectant formulations, establish a skin vitrification cryopreservation protocol, and verify its effectiveness.
Research Content and Methods:
Formulation and Process Optimization: Optimize cryoprotectant formulations based on the candidate molecules from Stage 2; test the permeability of protective agents and develop loading/unloading protocols.
Skin Cryopreservation and Evaluation: Design a skin vitrification cryopreservation procedure and evaluate the cryopreservation effect through cell viability tests, histological staining, and mechanical property analysis.
Stage Output: Efficient vitrification cryoprotectant formulation and optimized skin cryopreservation protocol.
[Suggested Illustration]: Schematic diagram of formulation optimization + skin tissue sections + mechanical property curves
Progressive Relationship
Stage 1 provides the "structure-property" design basis for Stage 2, Stage 2 provides the core functional molecules for Stage 3, and Stage 3 verifies the application effect of the entire process, forming a "mechanism-design-application" closed loop.
Illustration Style: Clear flowchart, with three stages distinguished by color, arrows indicating progressive logic, and key nodes accompanied by simplified schematic diagrams.](/_next/image?url=https%3A%2F%2Fpub-8c0ddfa5c0454d40822bc9944fe6f303.r2.dev%2Fai-drawings%2FXYJ0h2vTvP0v0DQtTMWj2YH0O11qgSqi%2Fdba97ac9-3cf1-42b8-9a8e-4074ebebd70b%2Fec20b4ed-f083-4abc-91f0-d73d16ffb130.png&w=3840&q=75)
